
Description
In the early days of DNA manipulation, DNA fragments were laboriously separated by gravity. Now gel electrophoresis is used as an efficient method for separation and analysis of macromolecules and their fragments, based on their size and charge. It is used in clinical chemistry, biochemistry and molecular biology to separate a mixed population of DNA and RNA fragments by length, to estimate the size of DNA and RNA fragments or to separate proteins. Gel electrophoresis apparatus usually consists of a variable voltage direct current power supply and a watertight box with two oppositely placed electrodes.